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gentamicin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gentamicin
    Gentamicin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gentamycin/Gentamicin+sulfate%2C+600+I%2EU%2E%2Fmg/pmc13240773-186-18-19
    Average 98 stars, based on 1 article reviews
    gentamicin - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Porcine β-defensin 5 (pBD-5) exhibits potential immunomodulatory effects in porcine gut epithelial cells, B-cells, and alveolar macrophages.
    Article Snippet: .. Immortalized swine B-cells (Murtaugh0001) derived from a pig with lymphoma were cultured and maintained in complete RPMI 1640 medium supplemented with 10 mM HEPES buffer, 1 mM non-essential amino acids, 1 mM sodium pyruvate, 50 μg/mL gentamycin, 5000 U/ mL penicillin-streptomycin, and 5% fetal bovine serum (all from Invitrogen, Mississauga, ON) (Rahe et al., 2020). .. Immortalized porcine macrophage cell line 3D4/31 was cultured and maintained in RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM non-essential amino acids, and 90% fetal bovine serum (Gibco Life Technologies, Co., Grand Island, NY, USA), according to supplier recommendations (ATCC, CRL− 2844).

    Article Title: Aquaporin-4–Specific T Cell Responses in NMOSD Revealed by mRNA-Engineered Dendritic Cells
    Article Snippet: .. CD14 + cells (monocyte derived dendritic cells (moDCs)) were cultured in X-Vivo 15 Media (Lonza, 02-060Q) with 800U GM-CSF and 250U IL-4 (PeproTech, AF-300-03, AF-200-04).For T cell feeding R10 Media was used adapted by Cimen Bozkus et al (2021) : RPMI 1640 Media was supplemented with HEPES (10mM), Gentamycin (0,1mg/mL), GlutaMax (1x) and Human Serum (10%) and supplemented with IL-2 (Gibco, PHC0027), IL-7(R &D Systems, BT-007-GMP-025) and IL-15 (PeproTech, 200-15). ..

    Cell Culture:

    Article Title: Porcine β-defensin 5 (pBD-5) exhibits potential immunomodulatory effects in porcine gut epithelial cells, B-cells, and alveolar macrophages.
    Article Snippet: .. Immortalized swine B-cells (Murtaugh0001) derived from a pig with lymphoma were cultured and maintained in complete RPMI 1640 medium supplemented with 10 mM HEPES buffer, 1 mM non-essential amino acids, 1 mM sodium pyruvate, 50 μg/mL gentamycin, 5000 U/ mL penicillin-streptomycin, and 5% fetal bovine serum (all from Invitrogen, Mississauga, ON) (Rahe et al., 2020). .. Immortalized porcine macrophage cell line 3D4/31 was cultured and maintained in RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM non-essential amino acids, and 90% fetal bovine serum (Gibco Life Technologies, Co., Grand Island, NY, USA), according to supplier recommendations (ATCC, CRL− 2844).

    Article Title: Aquaporin-4–Specific T Cell Responses in NMOSD Revealed by mRNA-Engineered Dendritic Cells
    Article Snippet: .. CD14 + cells (monocyte derived dendritic cells (moDCs)) were cultured in X-Vivo 15 Media (Lonza, 02-060Q) with 800U GM-CSF and 250U IL-4 (PeproTech, AF-300-03, AF-200-04).For T cell feeding R10 Media was used adapted by Cimen Bozkus et al (2021) : RPMI 1640 Media was supplemented with HEPES (10mM), Gentamycin (0,1mg/mL), GlutaMax (1x) and Human Serum (10%) and supplemented with IL-2 (Gibco, PHC0027), IL-7(R &D Systems, BT-007-GMP-025) and IL-15 (PeproTech, 200-15). ..

    Article Title: Differential modulation of glioma metabolism and the tumor microenvironment following dexamethasone and bevacizumab treatment
    Article Snippet: 167 168 Cell lines and reagents 169 Ac ce pte d M an us cri p on 16 June 2026 Maise et al, NOA-D-26-00145R1 12 Human glioma long-term cell line LN229 was obtained from ATCC (Wesel, DE), murine glioma 170 cell line GL261 was obtained from DSMZ (Wesel, DE) .. 172 All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) 173 supplemented with 10% fetal calf serum (FCS, Thermo Fisher Scientific) and 50 μg/ml 174 Gentamycin (Thermo Fisher Scientific) at 37°C and 5% CO2. .. 172 All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) 173 supplemented with 10% fetal calf serum (FCS, Thermo Fisher Scientific) and 50 μg/ml 174 Gentamycin (Thermo Fisher Scientific) at 37°C and 5% CO2.

    Article Title: Collagen VI is a fibrosis-associated signal disrupting muscle regeneration across distinct human myopathies.
    Article Snippet: Cell isolation, culture, and ECM production Human cells from muscle samples were isolated as described previously (Bigot et al, 2009): briefly, muscle biopsy samples were minced, and explants were plated onto dishes coated with fetal bovine serum (FBS) (Invitrogen). .. Isolated cells were cultured at 37 °C in growth medium (GM) consisting of 199 medium (Life Technologies) and Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies) at a 1:4 volume ratio supplemented with 20% FBS, 25 μg/mL fetuin (Life Technologies), 0.5 ng/ mL bFGF (Life Technologies), 5 ng/mL EGF (Life Technologies), 5 μg/mL insulin (Sigma-Aldrich), and 50 μg/mL gentamycin (Life Technologies) in a humid atmosphere containing 5% CO2 and tested for mycoplasma contamination. .. Myoblasts and FAPs were isolated by using magnetic activated cell sorting (MACS) with CD56 antibodycoupled microbeads (130-050-401, Miltenyi Biotec) according to the manufacturer’s instructions.

    Transfection:

    Article Title: Aberrant tau accumulation caused by MAPT mutations induces early pathological changes in axonal transport that are rescued by p38α inhibition.
    Article Snippet: Plasmids pcDNA3-TauWT(1N4R)-Flag-GFP11, pcDNA3-TauP301S(1N4R)-FlagGFP11, pHR- TauWT(1N4R)-Flag-GFP11 and pHR-TauP301S(1N4R)Flag-GFP11 were previously described75,76. pHR-SFFV-GFP1-10 (Addgene plasmid 80409; http://n2t.net/addgene:80409; RRID: Addgene_80409) and pHR-hSyn-EGFP (Addgene plasmid 114215; http://n2t.net/addgene:114215; RRID: Addgene_114215) were kind gifts from B. Huang (Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA, USA) and X. Han (Department of Biomedical Engineering, Boston University, Boston, MA, USA), respectively. pAAV-CamKIIα-BDNF-mCherry was assembled by PCR cloning from pJPA5-BDNF-mCherry (a gift of M. Verhage; Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, Amsterdam, the Netherlands) into pAAV-CaMKIIa-EGFP (a gift from B. Roth; Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Addgene plasmid 50469; http://n2t.net/addgene:50469; RRID: Addgene_50469). pAAV-CamKIIα-BDNF-mScarletI was assembled from LAMP1-mScarlet (a gift from D. Gadella; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, the Netherlands; Addgene plasmid 98827; http://n2t.net/addgene:98827; RRID: Addgene_98827) into pAAV-CamKIIα-BDNF-mCherry. pAAV-CamKIIα-Lamp1-mScarletI was assembled by inserting LAMP1-mScarletI into pAAV-CaMKIIa-EGFP. pcDNA3-MTBR-WT(1N4R)-Flag-GFP11 was assembled by inserting MTBR-4R into pcDNA3-TauWT(1N4R)-Flag-GFP11. pRK5-EGFP-Tau, pRK5-EGFP-Tau P301L, pRK5-EGFP-Tau AP-P301L and pRK5-EGFP-Tau E14-P301L were gifts from K. Ashe (Department of Neurology, University of Minnesota, Minneapolis, MN, USA) (Addgene plasmid 46904, http://n2t.net/addgene:46904, RRID: Addgene_46904; Addgene plasmid 46908, http://n2t.net/addgene:46908, RRID: Addgene_46908; Addgene plasmid 46906, http://n2t.net/addgene:46906, RRID: Addgene_46906; Addgene plasmid 46909, http://n2t.net/ addgene:46909, RRID: Addgene_46909). .. For lentivirus production, Lenti-X 293T cells (Takara Bio) were grown in DMEM supplemented with 10% fetal bovine serum, 1% L-glutamine and 0.1% gentamycin (Invitrogen) and transfected using Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s instructions. .. Viral particles were recovered using a Lenti-X Concentrator (Takara Bio) following the manufacturer’s instructions.

    Modification:

    Article Title: Mitochondrial NADK2-dependent NADPH controls tau oligomer uptake in human neurons.
    Article Snippet: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. .. Cat no. 632180) were grown in Dulbecco’s modified Eagle medium (DMEM)/F12 media (GIBCO) supplemented with HyClone cosmic calf serum (GEHealthcare) and 50 μg/ml gentamycin (GIBCO). ..

    Article Title: Differential modulation of glioma metabolism and the tumor microenvironment following dexamethasone and bevacizumab treatment
    Article Snippet: 167 168 Cell lines and reagents 169 Ac ce pte d M an us cri p on 16 June 2026 Maise et al, NOA-D-26-00145R1 12 Human glioma long-term cell line LN229 was obtained from ATCC (Wesel, DE), murine glioma 170 cell line GL261 was obtained from DSMZ (Wesel, DE) .. 172 All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) 173 supplemented with 10% fetal calf serum (FCS, Thermo Fisher Scientific) and 50 μg/ml 174 Gentamycin (Thermo Fisher Scientific) at 37°C and 5% CO2. .. 172 All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) 173 supplemented with 10% fetal calf serum (FCS, Thermo Fisher Scientific) and 50 μg/ml 174 Gentamycin (Thermo Fisher Scientific) at 37°C and 5% CO2.

    Article Title: Collagen VI is a fibrosis-associated signal disrupting muscle regeneration across distinct human myopathies.
    Article Snippet: Cell isolation, culture, and ECM production Human cells from muscle samples were isolated as described previously (Bigot et al, 2009): briefly, muscle biopsy samples were minced, and explants were plated onto dishes coated with fetal bovine serum (FBS) (Invitrogen). .. Isolated cells were cultured at 37 °C in growth medium (GM) consisting of 199 medium (Life Technologies) and Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies) at a 1:4 volume ratio supplemented with 20% FBS, 25 μg/mL fetuin (Life Technologies), 0.5 ng/ mL bFGF (Life Technologies), 5 ng/mL EGF (Life Technologies), 5 μg/mL insulin (Sigma-Aldrich), and 50 μg/mL gentamycin (Life Technologies) in a humid atmosphere containing 5% CO2 and tested for mycoplasma contamination. .. Myoblasts and FAPs were isolated by using magnetic activated cell sorting (MACS) with CD56 antibodycoupled microbeads (130-050-401, Miltenyi Biotec) according to the manufacturer’s instructions.

    Isolation:

    Article Title: Collagen VI is a fibrosis-associated signal disrupting muscle regeneration across distinct human myopathies.
    Article Snippet: Cell isolation, culture, and ECM production Human cells from muscle samples were isolated as described previously (Bigot et al, 2009): briefly, muscle biopsy samples were minced, and explants were plated onto dishes coated with fetal bovine serum (FBS) (Invitrogen). .. Isolated cells were cultured at 37 °C in growth medium (GM) consisting of 199 medium (Life Technologies) and Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies) at a 1:4 volume ratio supplemented with 20% FBS, 25 μg/mL fetuin (Life Technologies), 0.5 ng/ mL bFGF (Life Technologies), 5 ng/mL EGF (Life Technologies), 5 μg/mL insulin (Sigma-Aldrich), and 50 μg/mL gentamycin (Life Technologies) in a humid atmosphere containing 5% CO2 and tested for mycoplasma contamination. .. Myoblasts and FAPs were isolated by using magnetic activated cell sorting (MACS) with CD56 antibodycoupled microbeads (130-050-401, Miltenyi Biotec) according to the manufacturer’s instructions.



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